Nintendo TV Infinity

Super Mario World

 * Mario (Included in the Starter Pack.)
 * Luigi
 * Princess Toadstool (As a mission giver in the Playset.)
 * King Koopa (As a final boss.)

Donkey Kong Country

 * Donkey Kong (Included in the Starter Pack.)
 * Diddy Kong
 * King K. Rool (As a final boss.)

The Legend of Zelda

 * Link (Included in the Starter Pack.)
 * Zelda (As a mission giver in the Playset.)
 * Ganon (As a final boss.)

Kirby: Right Back at Ya!

 * Kirby (Included in the Kirby: Right Back at Ya! Play Set Pack)
 * King Dedede (Included in the Kirby: Right Back at Ya! Play Set Pack)
 * Meta Knight

Toy Box Exclusive

 * Pac-Man
 * Mega Man (Included in the Mega Man Toy Box Pack)
 * Dr. Wily (Included in the Mega Man Toy Box Pack)
 * Sonic (Included in the Adventures of Sonic the Hedgehog Toy Box Pack)
 * Tails (Included in the Adventures of Sonic the Hedgehog Toy Box Pack)

Super Mario World

 * Oogtar (As a mission giver in the playset, and unlockable in the Toy Box.)
 * Yoshi (As a ridable character, and unlockable in the Toy Box.)
 * Iggy "Hip" Koopa (As an enemy.)
 * Lemmy "Hop" Koopa (As an enemy.)
 * Wendy "Kootie Pie" Koopa (As an enemy.)
 * Larry "Cheatsy" Koopa (As an enemy.)
 * Roy "Bully" Koopa (As an enemy.)
 * Ludwig "Kooky" Von Koopa (As an enemy.)
 * Morton "Big Mouth" Koopa Jr. (As an enemy.)

Donkey Kong Country

 * Cranky Kong (As a mission giver in the playset, and unlockable in the Toy Box.)
 * Funky Kong (As a mission giver in the playset, and unlockable in the Toy Box.)
 * Candy Kong (As a mission giver in the playset, and unlockable in the Toy Box.)
 * Bluster Kong (As a mission giver in the playset, and unlockable in the Toy Box.)
 * Kritters (As enemies.)
 * Klump (As an enemy.)
 * Krusha (As an enemy.)
 * Inka Dinka Doo (Seen in cutscenes.)
 * Polly Roger (Seen in cutscenes.)

Voice cast

 * Fred Tataciore - Mario, Klump
 * Dan Castellaneta - Luigi, Pac-Man
 * Andrew Sabiston - Yoshi, Diddy Kong
 * Tracey Moore - Princess Toadstool
 * John Stocker - Oogtar
 * Kevin Michael Richardson - King Koopa
 * Tara Strong - Iggy "Hip" Koopa, Lemmy "Hop" Koopa
 * Tabitha St. Germain - Wendy "Kootie Pie" Koopa
 * Quinton Flynn - Larry "Cheatsy" Koopa, Morton "Big Mouth" Koopa Jr.
 * Dan Hennessey - Roy "Bully" Koopa
 * Michael Stark - Ludwig "Kooky" Von Koopa
 * Richard Yearwood - Donkey Kong
 * Michael Bell - Cranky Kong
 * Damon D'Oliveira - Funky Kong
 * Joy Tanner - Candy Kong
 * Donald Burda - Bluster Kong
 * Benedict Campbell - King K. Rool
 * Adrian Truss - Klump
 * Frank Welker - Krusha
 * Lawrence Bayne - Inka Dinka Doo
 * Rick Jones - Polly Roger
 * Amy Birnbaum - Kirby
 * Kerry Williams - Tiff
 * Grey Griffin - Tuff
 * Ted Lewis - King Dedede, Escargoon
 * Eric Stuart - Meta Knight
 * Ian James Corlett - Mega Man
 * Scott McNeil - Dr. Wily
 * Jaleel White - Sonic
 * Hayden Rolence - Tails